# Bubble plots on averages

For these data, a segmented bubble plot on a 2-d ordination provides a succinct summary of the relative balance of the main dietary components for these 7 fish species, when examined on data <u>averaged</u> over all replicate gut samples for each fish species (as in Fig. 7.16 of CiMC). On <ins>Data6</ins>, the standardised then transformed data sheet, take **Tools>Average**>(Samples•Averages for factor: <ins>species</ins>), giving a matrix of 7 samples (the 7 fish species) by 32 dietary categories, <ins>Data7</ins>. Compute Bray-Curtis similarities on this and produce the 2-d *n*MDS configuration, which has minimal stress and is therefore an excellent representation of the dietary dissimilarities among the fish species. The *means plot* below labels by the full species name and harmonises the symbols to a blue square. 

[![ScreenshotPage141b.png](https://learninghub.primer-e.com/uploads/images/gallery/2024-07/scaled-1680-/screenshotpage141b.png)](https://learninghub.primer-e.com/uploads/images/gallery/2024-07/screenshotpage141b.png)

Now, uncheck the History box on the **General** menu, remove the subtitle from the **Titles** tab and turn this into a segmented bubble plot by **Special>Main**>✓Bubble plot>(Worksheet: <ins>Data7</ins>), and **Change** variables to *Calanoid (copepods)*, *Polychaetes*, *Amphipods*, *Tanaids*, *Crabs*, *Cumaceans*, also increasing the Bubble scale. On the **Key** dialog, set a uniform range of 0-5 (square root scale, so the segment sizes in the key will represent an average 25% of diet by volume), change Boundary colour to white and make any other desired colour changes.  (If the names are required displaced from the bubble centres, or the key back-transformed to original units, key/titles moved etc., as in the equivalent Fig. 7.16 of CiMC for example, the plot can be copied and pasted to Powerpoint or other presentation software; this will be in vector not bitmap format so can readily be edited). Resave this fish diets workspace as <ins>WA fish ws</ins> for use in the next section, and close it. 

[![ScreenshotPage142a.png](https://learninghub.primer-e.com/uploads/images/gallery/2024-07/scaled-1680-/screenshotpage142a.png)](https://learninghub.primer-e.com/uploads/images/gallery/2024-07/screenshotpage142a.png)