# Table of Contents

- [**Citation**](https://learninghub.primer-e.com/link/662)
- [**Summary**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/chapter/summary)
   - [Introduction](https://learninghub.primer-e.com/link/950)
   - [New Statistical Methods in P8](https://learninghub.primer-e.com/link/952)
   - [New Tools & Utilities in P8](https://learninghub.primer-e.com/link/953)
- [**1. Expanded summary statistics**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/chapter/1-expanded-summary-statistics)
   - [1.1 Expansion from P7 to P8](https://learninghub.primer-e.com/link/955)
   - [1.2 Definitions of statistics](https://learninghub.primer-e.com/link/957)
   - [1.3 Biotic data: summary stats](https://learninghub.primer-e.com/link/958)
   - [1.4 Split summary stats results by groups](https://learninghub.primer-e.com/link/959)
   - [1.5 Environmental data: summary stats](https://learninghub.primer-e.com/link/960)
- [**2. Empirical distributions**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/chapter/2-empirical-distributions)
   - [2.1 What is an empirical distribution?](https://learninghub.primer-e.com/link/1020)
   - [2.2 Example: Empirical distributions of oyster sizes](https://learninghub.primer-e.com/link/1021)
- [**3. Dot plots and Violin plots**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/chapter/3-dot-plots-and-violin-plots)
   - [3.1 Plots of empirical densities](https://learninghub.primer-e.com/link/1022)
   - [3.2 Example: Dotplot of oyster sizes](https://learninghub.primer-e.com/link/1023)
   - [3.3 Example: Violin plot of kelp holdfast volumes](https://learninghub.primer-e.com/link/1024)
- [**4. Univariate non-parametric methods**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/chapter/4-univariate-non-parametric-methods)
   - [4.1 Wilcoxon signed-rank test](https://learninghub.primer-e.com/link/961)
   - [4.2 Example: Plankton hauls](https://learninghub.primer-e.com/link/967)
   - [4.3 Mann-Whitney U test](https://learninghub.primer-e.com/link/962)
   - [4.4 Example: Snapper in marine reserves](https://learninghub.primer-e.com/link/968)
   - [4.5 Kruskal-Wallis test](https://learninghub.primer-e.com/link/963)
   - [4.6 Example: A bivalve species from Ekofisk](https://learninghub.primer-e.com/link/969) 
   - [4.7 Kolmogorov-Smirnov test](https://learninghub.primer-e.com/link/964)
   - [4.8 Example: Sizes of oysters](https://learninghub.primer-e.com/link/970)
   - [4.9 Test of Association](https://learninghub.primer-e.com/link/965)
   - [4.10 Example: Ekofisk diversity](https://learninghub.primer-e.com/link/1018)
   - [4.11 Example: Associations between species](https://learninghub.primer-e.com/link/1019)
- [**5. New PERMANOVA Design file**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/chapter/5-new-permanova-design-file)
   - [Overview of new 'Design' options and tools](https://learninghub.primer-e.com/link/1025)
- [**6. Allow heterogeneous dispersions in PERMANOVA**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/chapter/6-allow-heterogeneous-dispersions-in-permanova)
   - [6.1 Overview - Allow heterogeneity](https://learninghub.primer-e.com/link/1034)
   - [6.2 ANOVA in a nutshell](https://learninghub.primer-e.com/link/1026)
   - [6.3 The Behrens-Fisher problem (BFP)](https://learninghub.primer-e.com/link/1027)
   - [6.4 Multivariate Behrens-Fisher problem](https://learninghub.primer-e.com/link/1028)
   - [6.5 Solutions to the multivariate BFP](https://learninghub.primer-e.com/link/1032)
   - [6.6 Example: one-way PERMANOVA allowing heterogeneity](https://learninghub.primer-e.com/link/1029)
   - [6.7 Heterogeneity in more complex designs](https://learninghub.primer-e.com/link/1033)
   - [6.8 Example: two-way crossed PERMANOVA allowing heterogeneity](https://learninghub.primer-e.com/link/1030)
- [**7. Finite factors**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/chapter/7-finite-factors)
   - [7.1 Overview - Finite factors](https://learninghub.primer-e.com/link/1036)
   - [7.2 Dichotomy: fixed vs random factors](https://learninghub.primer-e.com/link/1035)
   - [7.3 Not a dichotomy: a progression from fixed to random](https://learninghub.primer-e.com/link/1037)
   - [7.4 Example: environmental impact on molluscs](https://learninghub.primer-e.com/link/1038)
   - [7.5 Broader implications for detecting impact](https://learninghub.primer-e.com/link/1039)
- [**8. Specifying Subject/Whole-plot error in PERMANOVA**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/chapter/8-specify-subjectwhole-plot-error-in-permanova)
   - [8.1 Designs lacking replication](https://learninghub.primer-e.com/link/1040)
   - [8.2 Example: Split-plot - Woodstock vegetation](https://learninghub.primer-e.com/link/1042)
   - [8.3 Example: Repeated measures - Victorian avifauna](https://learninghub.primer-e.com/link/1041)   
- [**9. Group covariates**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/chapter/9-group-covariates)
   - [9.1 Why group covariables together?](https://learninghub.primer-e.com/link/1043)
   - [9.2 Periodic and cyclical models](https://learninghub.primer-e.com/link/1044)
   - [9.3 Example: Annual monthly cycles - B.C. macroalgae](https://learninghub.primer-e.com/link/1045)
- [**10. Centroid plots**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/chapter/10-centroid-plots)
   - [10.1 Ordinations for multi-factor designs](https://learninghub.primer-e.com/link/1046)
   - [10.2 Main effects plot](https://learninghub.primer-e.com/link/1047)
   - [10.3 Interaction plot](https://learninghub.primer-e.com/link/1048)
   - [10.4 Example: NZ fish assemblages](https://learninghub.primer-e.com/link/1049)
- [**11. Residual distances**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/chapter/11-residual-distances)
   - [11.1 What are 'residual' distances?](https://learninghub.primer-e.com/link/1050)
   - [11.2 Example: Plankton (revisited)](https://learninghub.primer-e.com/link/1051)
- [**12. Control charts**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/chapter/12-control-charts)
   - [12.1 Overview - Control charts](https://learninghub.primer-e.com/link/1054)
   - [12.2 Classical univariate control chart](https://learninghub.primer-e.com/link/1055)
   - [12.3 Classical multivariate control chart](https://learninghub.primer-e.com/link/1056)
   - [12.4 Bivariate normal example: NZ fish](https://learninghub.primer-e.com/link/1057)
   - [12.5 Dissimilarity-based multivariate control chart](https://learninghub.primer-e.com/link/1058)
   - [12.6 Additional notes on implementing control charts](https://learninghub.primer-e.com/link/1059)
   - [12.7 Example: Birds from Grand Forks](https://learninghub.primer-e.com/link/1060)
- [**13. New standardisation options**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/chapter/13-new-standardisation-options)
   - [13.1 Overview](https://learninghub.primer-e.com/link/1061)
   - [13.2 Analysing cumulative standardised data](https://learninghub.primer-e.com/link/1062)
   - [13.3 Example: Mussel sizes in the Gulf of Alaska](https://learninghub.primer-e.com/link/1063)
   - [13.4 Example: Gulf of Maine invertebrates - functional resemblance](https://learninghub.primer-e.com/link/1064)
- [**14. Create ordered groups**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/chapter/14-create-ordered-groups)
   - [14.1 Overview](https://learninghub.primer-e.com/link/1065)
   - [14.2 Example: NE Pacific groundfish vs depth](https://learninghub.primer-e.com/link/1066)
- [**15. Other new tools & utilities**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/chapter/15-other-new-tools-utilities)
   - [15.1 New default colour palette](https://learninghub.primer-e.com/link/1067)
   - [15.2 New selection options](https://learninghub.primer-e.com/link/1068)
   - [15.3 Re-name levels of a factor (or indicator)](https://learninghub.primer-e.com/link/1069)
   - [15.4 Add customised values/labels to graphical axes](https://learninghub.primer-e.com/link/1070)
   - [15.5 Split data sheet by factor/indicator](https://learninghub.primer-e.com/link/1071)
   - [15.6 Line plots for samples](https://learninghub.primer-e.com/link/1072)
   - [15.7 Output group-level stats from dispersion (or variability) weighting](https://learninghub.primer-e.com/link/1074)
   - [15.8 Output diagnostic plots from CAP](https://learninghub.primer-e.com/link/1075)
   - [15.9 New diagnostics for PCA/PCO plots](https://learninghub.primer-e.com/link/1076)
- [**16. Means plots**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/chapter/16-means-plots)
   - [16.1 A plot of means with error bars](https://learninghub.primer-e.com/link/1135)
   - [16.2 Example: Fal biota (one-way case)](https://learninghub.primer-e.com/link/1136)
   - [16.3 Example: Okura macrofauna (two-way nested case)](https://learninghub.primer-e.com/link/1137)
   - [16.4 Example: Leschenault fish (two-way crossed case)](https://learninghub.primer-e.com/link/1138)
   - [16.5 Example: New Zealand snapper (three-way case)](https://learninghub.primer-e.com/link/1139)
- [**References**](https://learninghub.primer-e.com/books/whats-new-in-primer-8/page/references)