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Forward and backward propagation
The Explorer tree not only makes it easy to navigate the sequence of steps taken in an analysis but also, importantly, reflects the program’s internal knowledge of the inter-relationships among data, resemblance sheets and plot windows. It uses this structure ...
Closing, redisplaying & tiling windows; Minimising windows; View menu
From the Explorer tree for the above illustration, you can see that workspace has been saved as Bristol Channel ws, with every row below this representing a window in the workspace, whether open or not. It is important to appreciate that whilst you can close d...
Understanding the Explorer tree
Understanding the Explorer tree, however, is the key to managing your work pattern in PRIMER. In the above workspace, click on the successive entries. After the workspace name ( Bristol Channel ws) two data matrices have clearly been opened into the workspa...
Rolling up branches of the tree
In the display of the Explorer tree at the beginning of this section, the SIMPROF1 window was prefaced by the rolled-up icon , and it is necessary to click on this to expand the branch below this point, replacing the rolled-up icon with one indicating t...
Renaming or deleting items in a workspace; Undo in the Explorer tree to reinstate or re-order
Met briefly in Section 1 and again above, renaming or deleting windows in the Explorer tree is an essential part of keeping a workspace navigable and understandable, especially since workspaces for many real analyses can become voluminous! Renaming is accompli...
Saving plots
The name of a window can also be changed as part of the process of saving it, as a file external to the workspace: if the file is given a different name in the act of saving it, then that new name will also replace the existing entry in the Explorer tree. Howe...
Vector vs. pixel plots
Closing the second PRIMER desktop and returning to the original Bristol Channel ws workspace, note the other options for saving a dendrogram or any plot file with File>Save Graph As>(Save as type: $\text{\hspace{3mm}}$ ). The vector format Windows Enhanced M...
Saving graph values; Saving results
Certain graphs, such as MDS ordinations (Section 8) or Cluster dendrograms, can be validly rotated in an infinity of ways (effectively), after the results window is generated, perhaps to align them better with a previous run under different transformation or c...
Adding notes
It is not permitted to edit directly the information in a results window. This tells you what operation or analysis was actually carried out, and what the outcome was, and should remain immutable, to avoid confusion if the workspace is revisited later. Natural...
Printing results and graphs
Direct printing from PRIMER is also possible for analysis endpoints such as results windows, all graphics windows, and notes (but not data sheets or resemblance matrices, which are generally too large and unwieldy for easy printing – selections of them are bes...
Automatic creation of multi-plots
The concept of a Multi-plot is a new feature in PRIMER 7. This construction has already been met in Section 4 where histograms of all selected environmental variables, using frequencies calculated over the samples, were presented in a single multi-plot window ...
User creation/ manipulation of multi-plots
In addition to automatic generation by certain routines, multi-plots can be created and populated by the user, to hold sets of related plots in a single composite window. File>New>(•Multi plot) gives a dialog box headed by a Graphs button which allows an order...
Plots menu
PRIMER 7 has a new Plots main menu, available when the active window is a data matrix. This brings together a number of standard plot formats, and more specialised ones, relevant to a range of multivariate analyses. Some are similar or identical to those in PR...
Workspace planning
To conclude this section, it is worth remarking that care taken in structuring workspaces will often pay dividends if the analysis results need to be returned to later. An Explorer tree represents a single workspace. It can contain several starting data matric...
Rationale for nMDS & mMDS
Chapter 5 of the CiMC methods manual describes the operation and rationale of multi-dimensional scaling (MDS) ordination, Analyse>MDS. The aim of MDS is to represent the samples as points in low-d space (often 2-d or 3-d, but PRIMER 7 will now compute MDS solu...
Combined MDS & ‘Fix Collapse’
A further new feature of nMDS in PRIMER 7 is the ability to minimise a combination of two stress functions, equally mixed – this has potential application, for example, to combining information on a common set of samples from community matrices (typically usin...
Diagnostic tools for MDS plots
In addition to the ability in a previous PRIMER version to Graph>Special>Overlays>(✓Overlay clusters) from a dendrogram onto a related MDS ordination, in order to judge agreement between these differing low-d displays of high-d data, PRIMER 7 now provides a wi...
Overlaying factors or other data (bubble plot)
The ability to display, on any ordination plot, external structure such as factors (e.g. for sites, times etc.) by Graph>Sample labels & symbols is a fundamental interpretational tool, as is the addition of time (or unidirectional space) trajectories, via Grap...
Running an nMDS (Exe nematodes)
From the directory C:\Examples v7\Exe nematodes, File>Open the workspace Exe ws, last seen in Section 6, of the sediment nematode communities at 19 inter-tidal sites in the Exe estuary. If this does not exist, open the data file Exe nematode abundance(.pri) in...
MDS results window
As with all results windows, the MDS1 window first lists the resemblance sheet on which analysis is carried out, and whether it was under any selection on the original sample set (given as ranges of sample numbers) but the main function of this window is to re...