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Change in Marine Communities

An Approach to Statistical Analysis and Interpretation, 3rd edition by K R Clarke, R N Gorley, P J Somerfield & R M Warwick (2014)

PERMANOVA+ for PRIMER: Guide to Software and Statistical Methods

M J Anderson, R N Gorley & K R Clarke (2008)

A Quick Guide to PRIMER

A quick guide to get you up and running with PRIMER software by Marti J. Anderson (2024)

PRIMER v7: User Manual / Tutorial

K. R. Clarke & R. N. Gorley (2015)

Should I use PRIMER or R?

Use both! They are good at different things. by Marti J. Anderson (2024)

Introduction and acknowledgements

Change in Marine Communities

Chapter 1: A framework for studying changes in community structure

Change in Marine Communities

Chapter 2: Simple measures of similarity of species ‘abundance’ between samples

Change in Marine Communities

Chapter 2

Chapter 3: Clustering methods

Change in Marine Communities

Chapter 4: Ordination of samples by principal components analysis (PCA)

Change in Marine Communities

Chapter 5: Ordination of samples by multi-dimensional scaling (MDS)

Change in Marine Communities

Chapter 6: Testing for differences between groups of samples

Change in Marine Communities

Chapter 7: Species analyses

Change in Marine Communities

Chapter 8: Diversity measures, dominance curves and other graphical analyses

Change in Marine Communities

Chapter 9: Transformations and dispersion weighting

Change in Marine Communities

Chapter 10: Species aggregation to higher taxa

Change in Marine Communities

Chapter 11: Linking community analyses to environmental variables

Change in Marine Communities

Chapter 12: Causality - community experiments in the field and laboratory

Change in Marine Communities

Chapter 13: Data requirements for biological effects studies - which components and attributes of the marine biota to examine?

Change in Marine Communities

Chapter 14: Relative sensitivities and merits of univariate, graphical/distributional and multivariate techniques

Change in Marine Communities

Chapter 15: Multivariate measures of community stress and relating to models

Change in Marine Communities

Chapter 16: Further multivariate comparisons and resemblance measures

Change in Marine Communities

Chapter 17: Biodiversity and dissimilarity measures based on relatedness of species

Change in Marine Communities

Chapter 18: Bootstrapped averages for region estimates in multivariate means plots

Change in Marine Communities

Appendices

Change in Marine Communities

0.1 Introduction

Change in Marine Communities Introduction and acknowledgements

Third edition The third edition of this unified framework for non-parametric analysis of multivariate data, underlying the PRIMER software package, has the same form and similar chapter headings to its predecessor (with an additional chapter). However, the tex...

0.2 Acknowledgements

Change in Marine Communities Introduction and acknowledgements

Any initiative spanning quite as long a period as the PRIMER software represents (the first recognisable elements were committed to paper over 30 years ago) is certain to have benefited from the contributions of a vast number of individuals: colleagues, studen...

1.1 Introduction

Change in Marine Communities Chapter 1: A framework for studying cha...

The purpose of this opening chapter is twofold: a) to introduce some of the data sets which are used extensively, as illustrations of techniques, throughout the manual; b) to outline a framework for the various possible stages in a community analysis¶. Example...

1.2 Univariate techniques

Change in Marine Communities Chapter 1: A framework for studying cha...

For diversity indices and other single-variable extractions from the data matrix, standard statistical methods are usually applicable and the reader is referred to one of the many excellent general statistics texts (e.g. ). The requisite techniques for each s...

1.3 Example: Frierfjord macrofauna

Change in Marine Communities Chapter 1: A framework for studying cha...

The first example is from the IOC/GEEP practical workshop on biological effects of pollutants (), held at the University of Oslo, August 1986. This attempted to contrast a range of biochemical, cellular, physiological and community analyses, applied to field ...

1.4 Distributional techniques

Change in Marine Communities Chapter 1: A framework for studying cha...

Table 1.3. Distributional techniques. Summary of analyses for the four stages. A less condensed form of diversity summary for each sample is offered by distributional/graphical methods, outlined for the four stages in Table 1.3. Representation is by curves ...

1.5 Example: Loch Linnhe macrofauna

Change in Marine Communities Chapter 1: A framework for studying cha...

Table 1.4. Loch Linnhe macrofauna {L}. Abundance/biomass matrix (part only); one (pooled) set of values per year (1963–1973). Fig. 1.3. Loch Linnhe and Loch Eil, Scotland {L}. Map of site 34 (Linnhe) and site 2 (Eil), sampled annually over 1963–1973. de...

1.6 Example: Garroch Head macrofauna

Change in Marine Communities Chapter 1: A framework for studying cha...

describe the sampling of a transect of 12 sites across the sewage-sludge disposal ground at Garroch Head in the Firth of Clyde, SW Scotland ({G}, Fig. 1.5). The samples considered here were taken during 1983 and consisted of abundance and biomass values of 8...

1.7 Multivariate techniques

Change in Marine Communities Chapter 1: A framework for studying cha...

Table 1.5 summarises some multivariate methods for the four stages, starting with three descriptive tools: hierarchical clustering (agglomerative or divisive), multi-dimensional scaling (MDS, usually non-metric) and principal components analysis (PCA). Table 1...

1.8 Example: Nutrient enrichment experiment, Solbergstrand

Change in Marine Communities Chapter 1: A framework for studying cha...

Table 1.7. Nutrient enrichment experiment, Solbergstrand mesocosm, Norway {N}. Meiofaunal abundances (shown for copepods only) from four replicate boxes for each of three treatments (Control, Low and High levels of added nutrients). Fig. 1.12. Nutrient en...

1.9 Summary

Change in Marine Communities Chapter 1: A framework for studying cha...

A framework has been outlined of three categories of technique (univariate, graphical/distributional and multivariate) and four analysis stages (representing communities, discriminating sites/conditions, determining levels of stress and linking to environmenta...

2.1 Similarity for quantitative data matrices

Change in Marine Communities Chapter 2: Simple measures of similarit...

Data matrix The available biological data is assumed to consist of an array of p rows (species) and n columns (samples), whose entries are counts or densities of each species for each sample, or the total biomass of all individuals, or their percentage cover, ...

2.2 Example: Loch Linnhe macrofauna

Change in Marine Communities Chapter 2: Simple measures of similarit...

A trivial example, used in this and the following chapter to illustrate simple manual computation of similarities and hierarchical clusters, is provided by extracting six species and four years from the Loch Linnhe macrofauna data {L} of , seen already in Fig....

2.3 Presence/absence data

Change in Marine Communities Chapter 2: Simple measures of similarit...

As discussed at the beginning of this chapter, quantitative uncertainty may make it desirable to reduce the data simply to presence or absence of each species in each sample, or this may be the only feasible or cost-effective option for data collection in the ...

2.4 Species similarities

Change in Marine Communities Chapter 2: Simple measures of similarit...

Starting with the original data matrix of abundances (or biomass, area cover etc), the similarity between any pair of species can be defined in an analogous way to that for samples, but this time involving comparison of the ith and lth row (species) across all...

2.5 Dissimilarity coefficients

Change in Marine Communities Chapter 2: Simple measures of similarit...

The converse concept to similarity is that of dissimilarity, the degree to which two samples are unlike each other. As previously stated, similarities (S) can be turned into dissimilarities ($\delta$), simply by: $$ \delta = 100 -S \tag{2.11} $$ which of cou...

2.6 More on resemblance measures

Change in Marine Communities Chapter 2: Simple measures of similarit...

On the grounds that it is better to walk before you try running, discussion of comparisons between specific similarity, dissimilarity and distance coefficients, that the PRIMER software refers to generally by the term resemblance measures, is left until after ...

3.1 Cluster analysis

Change in Marine Communities Chapter 3: Clustering methods

The previous chapter has shown how to replace the original data matrix with pairwise similarities, chosen to reflect the particular aspect of community similarity of interest for that study (similarity in counts of abundant species, similarity in location of r...

3.2 Hierarchical agglomerative clustering

Change in Marine Communities Chapter 3: Clustering methods

The most commonly used clustering techniques are the hierarchical agglomerative methods. These usually take a similarity matrix as their starting point and successively fuse the samples into groups and the groups into larger clusters, starting with the highes...

3.3 Example: Bristol Channel zooplankton

Change in Marine Communities Chapter 3: Clustering methods

perform hierarchical cluster analyses of zooplankton samples, collected by double oblique net hauls at 57 sites in the Bristol Channel UK, for three different seasons in 1974 {B}. This was not a pollution study but a baseline survey carried out by the Plymo...